```r
t.test(Site1,Site2)
```

```
## 
## 	Welch Two Sample t-test
## 
## data:  Site1 and Site2
## t = -2.283, df = 47.762, p-value = 0.02692
## alternative hypothesis: true difference in means is not equal to 0
## 95 percent confidence interval:
##  -15.924396  -1.009143
## sample estimates:
## mean of x mean of y 
##  100.7692  109.2360
```